Cancer :: Turning a cellular sentinel into a cancer killer

Howard Hughes Medical Institute researchers have developed two strategies to reactivate the p53 gene in mice, causing blood, bone and liver tumors to self-destruct.

Inactivation of p53 can set the stage for the development of different types of cancer. The researchers’ findings show for the first time that inactivating the p53 gene is necessary for maintaining tumors. While the researchers caution that cancers can mutate to circumvent p53 reactivation, they believe their findings offer ideas for new approaches to cancer therapy.

The research was carried out independently by two Howard Hughes Medical Institute (HHMI) research teams led by Tyler Jacks at the Massachusetts Institute of Technology and Scott Lowe at Cold Spring Harbor Laboratory. Both papers were published online January 24, 2007, in advance online publication articles in the journal Nature.Although researchers have long known that p53 inactivation plays a central role in the development of cancer, little was known about whether p53 inactivation played a role in maintaining cancers. And researchers were not sure whether switching p53 back on in tumor cells would have any therapeutic effect.

“It had been demonstrated that overexpressing p53 at very high levels could arrest or kill tumors, said Lowe. “But at such high levels, p53 might not be working through a physiological mechanism. So, it was an open question whether restoring the p53 pathway would have any anti-tumor effect.” For one thing, the high mutation rate in cancers might enable a cancer to switch the p53 pathway back off, or to circumvent the pathway in some other fashion. For those reasons, researchers were not sure whether the pathway would be a useful therapeutic target.

To reactivate p53, Lowe and his colleagues used a genetic technique they had developed to induce an aggressive form of liver cancer in mice. Although they had inactivated p53 in the mice, they genetically engineered the mice so that they could reverse p53 inactivation by giving the animals the antibiotic doxycycline. They suppressed p53 protein levels by using RNA interference (RNAi) that had been modified so that RNAi could be switched off by the antibiotic. The RNA interference technology was developed in collaboration with HHMI investigator Gregory Hannon at Cold Spring Harbor Laboratory.

When the researchers reactivated p53 in the mice they found that the liver tumors completely disappeared. “This was quite surprising,” said Lowe. “We were working with a very advanced, aggressive tumor, but when we reestablished p53, not only did it stop growing, it went away.

“But the second surprise?and perhaps the more scientifically interesting one?was why the tumor went away,” said Lowe.

“We expected the tumor cells to undergo programmed cell death, or apoptosis. But instead, we saw evidence for a very different process that p53 also regulates?senescence, or growth arrest. What really excited us was evidence that this senescence somehow triggered the innate immune system to kill the tumor cells.” Involvement of the innate immune system suggests there may be an unknown mechanism by which cancers can trigger the immune system, he said. Lowe and his colleagues are now exploring how the innate immune system might be enlisted against cancer.


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